Plasmocitoma extramedular em bulbo peniano de cão: relato de caso / Extramedullary plasmacytoma in the penile bulb of a dog: case report

O objetivo deste relato de caso é descrever a ocorrência de plasmocitoma em bulbo peniano de um cão, classificado como uma doença extramedular não cutânea de localização rara e casuística inédita. Um cão, sem raça definida, com sete anos de idade e pesando 15kg, não castrado, apresentou histórico clínico de anorexia, vômitos, anúria e constipação. Ao exame específico da genitália externa, foi encontrada uma massa em bulbo peniano durante a inspeção do prepúcio, aderida à pele e encapsulada, extremamente firme e arredondada, medindo cerca de 6cm de diâmetro. Por meio da ultrassonografia dessa estrutura, foi observado aumento do volume regional com ecotextura heterogênea e ecogenicidade mista, além de neovascularização tecidual ao Doppler colorido. Foi realizada biópsia da massa, sendo verificada a presença de neoplasia de células redondas. A caracterização do tumor foi realizada pela imuno-histoquímica, e as células neoplásicas foram imunoexpressas para CD79a e MUM1, indicando o diagnóstico de plasmocitoma extramedular. Embora os tumores penianos em cães sejam os predominantemente venéreos transmissíveis (TVT), e os plasmocitomas sejam neoplasias raras nessa localização, este relato de caso fornece com ineditismo a ocorrência de plasmocitoma extramedular em bulbo peniano de cão, condição ainda não descrita em veterinária.

Non-cutaneous extramedullary plasmacytomas are relatively rare in dogs, affecting mainly the oral cavity and bowel loops. The involvement of the penile bulb has not been described, a fact of great importance for obstetric and veterinary oncology. The aim of this case report is to describe the occurrence of plasmacytoma in a dog's penile bulb, classified as a non-cutaneous extramedullary disease of rare location and unpublished casuistry. A non castrated dog of undefined breed, with seven years of age and weighing 15kg, presented clinical history of anorexia, vomiting, anuria and constipation. By specific examination of the external genitalia, a penile bulb mass was found in the preputial inspection, which was adhered to the encapsulated skin, extremely firm and rounded, measuring approximately 6 cm in diameter. By ultrasound evaluation of the structure in the penile bulb an increase of regional volume with heterogeneous echotexture and mixed echogenicity and tissue neovascularization upon color Doppler was observed. Incisional biopsy of the mass was performed and showed the presence of neoplasia of round cells. The characterization of the tumor was performed by immunohistochemistry and the neoplastic immuno cells were expressed CD79a and MUM1, indicating the diagnosis of extramedullary plasmacytoma. Although the penile tumors in dogs are predominantly transmissible venereal tumors (TVT) and plasmocytomas are rare neoplasms in this location, this case report provides a novel occurrence of extramedullary plasmacytoma in the penile bulb of a dog, a condition not yet described in veterinary.

Detecção de DNA de Brucella spp. em amostras de sangue e de suabe vaginal ou prepucial de cães do município de Natal, Rio Grande do Norte, Brasil / Detection of Brucella spp. DNA in samples of blood and vaginal or preputial swab from dogs in the county of Natal, Rio Grande do Norte, Brazil

Objetivou-se com este trabalho detectar o DNA de Brucella spp. em amostras de sangue e de suabe vaginal ou prepucial de 80 cães sorologicamente positivos para brucelose pela prova de imunodifusão em gel de ágar (IDGA), no município de Natal, estado do Rio Grande do Norte, Brasil. Amostras de sangue total foram colhidas com anticoagulante (citrato de sódio) juntamente com amostras de suabe vaginal e prepucial , para extração de DNA e posterior realização da rea- ção em cadeia da polimerase (PCR) empregando-se os primers ITS66 e ITS279. O DNA de Brucella spp. foi amplificado em seis animais, sendo um animal em ambas as amostras, dois cães em amostras de sangue e três em amostras de suabe do trato reprodutivo. Concluiu-se que a infecção por Brucella spp. está presente em cães no município de Natal, e que a detecção de DNA do agente em amostras de suabe do trato reprodutivo podem ser utilizadas como ferramenta suple- mentar no diagnóstico de brucelose canina.

The aim of this work was to detect Brucella spp. DNA in samples of blood and vaginal or preputial swabs in 80 sero-positive dogs for brucellosis by agar gel immunodiffusion test (AGID) from the county of Natal, Rio Grande do Norte State, Brazil. Whole blood samples were collected with anticoagulant (sodium citrate) and vaginal and preputial swab samples for DNA extraction and polymerase chain reaction (PCR) employing ITS66 and ITS279 primers. Six animals showed amplification of Brucella spp., being one animal in both samples, two dogs only in blood samples, and three only in reproductive tract swabs. It is concluded that infection due to Brucella spp. occurs in dogs from the county of Natal, and the detection of DNA of the agent in reproductive tract swabs may be used as complementary tool in the diagnosis of canine brucellosis.

Congenital megaprepuce: a new alternative technique for surgical correction

OBJECTIVE: To present a new alternative technique for surgical treatment of congenital megaprepuce. MATERIALS AND METHODS: From April 2004 to April 2006, five patients aged 2 to 5 years were treated using the new technique. The technique is described and illustrated. It differs from other techniques in that it takes into consideration the constant ballooning of the foreskin, which gives to the external genitalia the aspect of a penoscrotal transposition. Cosmetic and functional success were also assessed by a case review. RESULTS: After a follow-up of 1 to 3 years, all patients have normal voiding and a satisfactory cosmetic aspect. CONCLUSION: This new technique could be a useful alternative in treatment of the congenital megaprepuce.